Cell & Developmental Biology of Xenopus: Gene Discovery & Disease

March 30 - April 12, 2022
Applications Due: January 31

Instructors
Lance Davidson, University of Pittsburgh
Chenbei Chang, University of Alabama at Birmingham

2022 Lecturers
Hiro Funabiki, The Rockefeller University
Douglas Houston, University of Iowa
Mustafa Khokha, Yale University
Carole LaBonne, Northwestern University
Karen Liu, King’s College London, UK
Roberto Mayor, University College London, UK
Rachel Miller, University of Texas
Brian Mitchell, Northwestern University
Gert Jan Veenstra, Radboud University, Netherlands
Sarah Woolner, University of Manchester, UK
Martin Wuhr, Princeton University

In vivo animal models are an important tool for the understanding of human development and disease. Studies using the frog Xenopus have made remarkable contributions to our understanding of fundamental processes such as cell cycle regulation, transcription, translation and many other topics. Xenopus is remarkable for studying development and disease, including birth defects, cancer, and stem cell biology. Because Xenopus are easy to raise, producing many thousands of eggs per day, these frogs have emerged as a premiere model for understanding of human biology from the fundamental building blocks to the whole organism. The recent development of CRISPR/Cas9 technology has made it easy to target genes of interest using Xenopus. This course has been designed with that in mind. Our goal is for each student to design a set of experiments focusing on their gene or biological interest. Prior to starting the course, students will be expected to choose gene(s) of interest, and the instructors will generate sgRNAs targeting these genes. These can be the students’ own genes, or chosen from a bank provided by the instructors. The gene targeting experiments will be combined with other manipulations, such as tissue explants and transplants and live imaging to analyze the function of the genes.

For more information and to apply please visit the Cell & Developmental Biology of Xenopus: Gene Discovery & Disease course website.

https://meetings.cshl.edu/courses.aspx?course=C-XENO&year=22

Dear Xenopus Community

Sadly I am writing to inform you that we will have to cancel the Bioinformatics Workshop that was to be held in early May. This is of course due to the coronavirus and issues with travel, especially for international travel, which includes the main Instructor. We hope to offer this workshop in the near future

Marko

Marko Horb, Ph.D. |Director, National Xenopus Resource (NXR) |Senior Scientist, Bell Center for Regenerative Biology and Tissue Engineering
Marine Biological Laboratory | 7 MBL Street | Woods Hole, MA 02543

Dear Xenopus community

Please consider applying for the Embryology course that is held each summer here at the MBL. Deadline has been extended.

Marko

Woods Hole Embryology Course Applications Due February 11, 2020

The application deadline has been extended for the Embryology Course at the Marine Biological Laboratory in Woods Hole. This fantastic course has been offered each summer since 1893. Students receive very unique and broad training in animal developmental biology, covering numerous and diverse species. In addition, they are able to directly interact with top scientists from around the world in the field of developmental biology, establishing lifetime scientific networks.

The course runs this year from May 31 – July 13, 2020 Course directors: Dave Sherwood (Duke) and Carole LaBonne (Northwestern).

Please encourage your students and post-docs to apply - former students of this course uniformly say that it had a tremendous impact on their career. Don’t be off-put by the sticker price - significant financial aid is available.

Course webpage: https://www.mbl.edu/embryology


Marko Horb, Ph.D. |Director, National Xenopus Resource (NXR) |Senior Scientist, Bell Center for Regenerative Biology and Tissue Engineering
Marine Biological Laboratory | 7 MBL Street | Woods Hole, MA 02543

Dear Xenopus Community,

Registration is now open for the next Bioinformatics Workshop that is being held May 3-9, 2020. You can always select the pay later option if you don’t want to pay right away. For more details please visit the website (https://www.mbl.edu/xenopus/workshops/bioinformatics).

Marko


Marko Horb, Ph.D. |Director, National Xenopus Resource (NXR) |Senior Scientist, Bell Center for Regenerative Biology and Tissue Engineering
Marine Biological Laboratory | 7 MBL Street | Woods Hole, MA 02543

Hello everyone,

I am writing to inform you that the application deadline for the CSHL Xenopus course has been extended to Feb 11 (next Tuesday). I highly encourage you to ask your postdoctoral fellows or students to apply for the course. The course covers a variety of topics and techniques, such as CRISPR-Cas9-mediated genome editing, oocyte manipulation, embryo injection, tissue dissections (mesoderm, neural crest, organ primordia, etc.), in situ hybridization and antibody staining, in vitro and in vivo imaging, concepts of -omics, cell biological studies using Xenopus, organogenesis, regeneration, models for human diseases, etc. The students are encouraged to bring their own projects to the course, and we will provide one sgRNA to each student. There are also a limited number of free antisense morpholino oligos available for several selected projects. The course starts March 25 and ends April 7. If you need additional information and/or want to apply for the course, here is the link to the course website:

https://meetings.cshl.edu/courses.aspx?course=c-xeno&year=20

Thanks for your help in promoting the course.

All the best,
Lance and Chenbei

----------------------------------------------------------------------

Dear Colleagues,

Greetings and salutations to all.

We are asking for your assistance to promote the 2020 Xenopus Course at Cold Spring Harbor. The Xenopus course at CSH has a long tradition of training the next generation of Xenopus researchers in critical skills to exploit Xenopus for understanding cell and developmental biology. Stipends are available to offset tuition costs (see the website for details).

To get the most out of the course, we encourage students to bring their own projects. Students are encouraged to identify genes of interest, we will design sgRNAs for these genes, and then students can knock these genes out at the course and analyze any phenotypes using all of the tools available from advanced high-speed fluorescent confocal imaging to cut-and-paste embryology.

In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSH. We plan to build interactions between our groups to image Xenopus embryos using the latest imaging methods. This was a huge success last year and offers the possibility to try light-sheet, high-speed live confocal, and super-resolution imaging methods.

Important Dates:
Course – March 25-April 7, 2020
Application Due Date: January 31, 2020

We have an exciting list of speakers/instructors including:

Chenbei Chang, University of Alabama;
Lance Davidson, University of Pittsburgh;
Rebecca Heald, University of California at Berkeley;
Douglas Houston, University of Iowa;
Mustafa Khokha, Yale University;
Ray Keller, University of Virginia;
Roberto Mayor, University College London, UK;
Bryan Mitchell, Northwestern University;
Nanette Nascone-Yoder, North Carolina State University;
Andrea Wills, University of Washington;
Sarah Woolner, University of Manchester, UK;
Martin Wuhr, Princeton University

For more information and application submission, please visit the website.


Thanks,
Chenbei Chang, University of Alabama
Lance Davidson, University of Pittsburgh


Course objectives:

Microinjection, microdissection, and grafts - Organizer, animal cap, neural crest and more. In situ hybridization, immunohistochemistry, CRISPR, and MO loss of function.
Germ cell isolation and transplants. Live cell and embryo Imaging and fluorescence - confocal microscopy, image analysis and figure design. Genomic and proteomic approaches to development, regeneration, and disease models.

ナショナルバイオリソースプロジェクト（NBRP）・ネッタイツメガエルでは、広島大学両生類研究センターにおいて、下記の日程で実験技術講習会を開催いたします。主にネッタイツメガエルの使用に関して初心者の方を対象とした内容を予定しております。本リソースを使ってみたい方は、ぜひご参加下さい。

日程：2020年3月3日（火曜日）〜3月5日（木曜日）
講習会の概要
　　　　　　　　　　ネッタイツメガエルの概要
　　　　　　　　　　ゲノム配列情報の利用講習
　　　　　　　　　　飼育法・人工授精・マイクロインジェクション
　　　　　　　　　　などの座学と実技講習

詳細・申し込みは下記講習会HPよりお願い致します。
https://sites.google.com/view/tropnbrp202003/%E3%83%9B%E3%83%BC%E3%83%A0

講習会の参加費は無料ですが、宿泊費、旅費は各自参加者がご負担ください。宿泊施設に関しては、広島大学の宿泊施設（学士会館と山中会館、いづれもシングル）をご用意しております。どちらも部屋数に限りがあります。参加申込時にご希望をご連絡ください。

参加申し込み締め切りは２月３日です。もし参加希望者数が講習会定員を超える場合は、先着順にさせていただきます。また年度末で忙しく、興味はあるが参加はできない、あるいは特定の技術等について講習を受けたいという方がおられましたら別途ご相談ください。調整の上、別日程で個別講習を行うことも検討させていただきます。


両生類研究センター　センター長　荻野肇

技術講習会担当：古野伸明、井川武、鈴木誠、鈴木菜花

Dear Xenopus Community
The 5th Genome Editing Workshop will take place Oct 14-24. Registration is now open. Please visit the webpage for details (https://www.mbl.edu/xenopus/workshops/genomeediting-workshop). The syllabus is now posted. A few highlights that might be of interest.
1. Costs for this workshop are only $750 for a shared room and $1300 for a single room. Financial support for the workshop comes from the NIH, NXR and NSF.
a. YES that is correct $750 for 10 days at the MBL as part of this awesome genome editing workshop. You won’t find a better deal. BUT space is limited

2. All room and board is funded by Participant Support Costs from the NSF EDGE grant awarded to Gary Gorbsky and myself. This is the final year of this Participant Support cost.

3. NEW for 2019!!! Travel support is now being offered for each participant up to $1000. Yes that is correct funds are available for travel as well.

4. The course includes new hands on training for working with the new euploid Xenopus cell lines recently created by Gary. You will learn how to work with the cell lines and have the opportunity to generate knockin cell lines during the course and can take the cell lines home afterwards. (You will have to make the donor template in advance.)

5. The course costs cover the primers and sgRNA and other reagents for making mutants in your gene of interest.
a. The NXR will grow your own founder lines to adulthood as part of this course. The last day is focused on providing individuals the time to inject embryos for founders, provided they show successful cutting with the designed sgRNA

6. The course is limited to 15 individuals. So register as soon as possible to take advantage of this great price.

Instructors for this workshop are:
Gary Gorbsky
Rachel Miller
Doug Houston
Thomas Naert (new- cancer models and base editing)
Marcin Wlizla
Hitoshi Yoshida
Nikko Shaidani
Marko Horb


Are any of you interested? Sign up now!! Or email me for more information. You won’t get a better price for such a course. Do you have a student, postdoc or technician you want to gain hands on experience with genome editing in Xenopus? Send them!! Don’t wait otherwise spaces will fill up.

Look forward to seeing you.

Marko

ナショナルバイオリソースプロジェクト（NBRP）・ネッタイツメガエルでは、今年も広島大学両生類研究センターにおいてゲノム編集実験技術講習会を開催いたします。概要を以下に記しますので、奮ってご参加をご検討下さい。なお、今回の講習では、始めに当方から実験方法の説明とデモをおこなった後、参加者が自由に繰り返し実験をおこない、確実に技術をマスターしていただく形式を予定しております。


講習内容
・CRISPR/Cas9法による遺伝子の破壊実験をおこないます。
　当方でtyrosinase遺伝子に対するsgRNAとCas9を用意いたします。
　ご希望の方にはGFPノックインベクターも提供いたします。
・ご自分の研究対象とする遺伝子のsgRNAを用いたいというご希望がありましたら、
　ご相談に応じますのでご連絡下さい。本学の組換えDNA実験規定で対応できるか検討いたします。
・CRISPR/Cas9法の代わりにRNAインジェクション実験をおこなうことも可能です。
　操作した生体のお持ち帰りをご希望の方は、該当機関の間で手続きが必要となりますので、事前にご連絡ください。

日程
・2019年９月17日(火) 午後〜2019年９月19日(木) 午後
　参加者のご希望により20日(金)まで延長可能です。
・「別日程で遺伝子破壊実験等について指導を後日受けたい」という方がおられましたら、
　次回の講習会あるいは個別指導等についてご相談に応じますのでご連絡ください。

宿泊費，旅費等
・宿泊費，旅費は各自参加者にご負担いただきますが、講習会参加費は無料です。
・宿泊施設に関しては、
　広島大学の学士会館（3,800円/一泊）と山中会館（2,000円/一泊）のお部屋（いずれもシングル）をご用意しておりますが、
　学士会館は部屋数に限りがあります。参加申し込み時にご希望をご連絡ください。

定員
・全体で３組、６名くらいまで（インジェクターの設置数に対応）。
・ご連絡いただいた方々から、状況を鑑み、今回ご参加いただけるか当方からご連絡いたします。
　今回ご参加いただけなかった方々には、できるだけ次回の講習会あるいは個別指導等でご対応させていただきますので、何卒ご了承下さい。
・独立に実験されるグループで３組とさせていただきます（例：教員が院生１、２名と参加される場合は１組）。
　１グループ３人以上でのご参加を希望される場合は対応を検討いたしますのでご連絡下さい。

参加申し込みの締め切りは８月23日(金）とさせていただきます。
参加ご希望の方は、以下の参加フォームに記入して、
nfuruno@hiroshima-u.ac.jp（古野）までご返信してください。


両生類研究センター　センター長　荻野肇
　技術講習会担当：古野伸明、井川武、鈴木誠、鈴木菜花


参加申し込みフォーム
＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿
　参加代表者者の氏名
　同伴参加者の氏名
　所属
　住所
　代表者の連絡先（E-mailと電話番号）
　宿泊希望施設と部屋数（学士会館または、山中会館）
　ご希望の宿泊期間（９月17日〜19日あるいは20日）
　希望実験内容（tyrosinase sgRNAを利用、sgRNAなどを持ち込み希望の場合は遺伝子名を記載して下さい。）
＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿＿

日程の概要：
９月17日（火）12：00〜13：00　講習会参加者受付
　　　　　　　13：00〜13：20　宿泊案内等の事務手続きの説明
　　　　　　　13：20〜14：20　組換えDNA講習（本学規定）
　　　　　　　14：30〜15：00　休憩
　　　　　　　15：00〜17：30　CRISPR/Cas9法の技術講習
　　　　　　　17：30〜 宿泊施設（学士会館）へ案内
９月18日（水）CRISPR/Cas9法の技術講習
９月19日（木）CRISPR/Cas9法の技術講習（午後に終了予定）
９月20日（金）希望者はこの日まで実験を継続、午後に終了。

Dear Xenophiles,

The 5th Genome Editing Workshop will be held Oct 14-24. Registration is now open for this workshop:
https://mbl.ungerboeck.com/prod/emc00/register.aspx?OrgCode=10&EvtID=15205&AppCode=REG&CC=119042503651

More information regarding the course can be found on the NXR webpage
https://www.mbl.edu/xenopus/workshops/genomeediting-workshop

We look forward to seeing you there.

Marko

アラバマ大学のChenbei Chang博士から2019 Xenopus Course at Cold Spring Harborの案内が届いていますので、転載いたします。

広島大学両生類研究センター　鈴木 厚


Dear Colleagues,

Greetings and salutations to all.
We are asking for your assistance to promote the 2019 Xenopus Course at Cold Spring Harbor. The Xenopus course at CSH has a long tradition of training the next generation of Xenopus researchers in critical skills to exploit Xenopus for understanding cell and developmental biology. To get the most out of the course, we encourage students to bring their own projects. Students are encouraged to identify genes of interest, we will design sgRNAs for these genes, and then students can knock these genes out at the course and analyze any phenotypes using all of the tools available from advanced high-speed fluorescent confocal imaging to cut-and-paste embryology.
In addition, we have the good fortune of overlapping with the Quantitative Imaging (QI) course at CSH. We plan to build interactions between our groups to image Xenopus embryos using the latest imaging methods. This was a huge success last year and offers the possibility to try light-sheet, high-speed live confocal, and super-resolution imaging methods.

Important Dates:
Course - April 2-16, 2019
Early Due Date: Dec. 1, 2018 (already passed)
Application Due Date: January 31, 2019

We have an exciting list of speakers/instructors including:

Sang-Wook Cha, University of Central Missouri;
Chenbei Chang, University of Alabama;
Ken Cho, University of California at Irvine;
Frank Conlon, University of North Carolina;
Lance Davidson, University of Pittsburgh;
Rebecca Heald, University of California at Berkeley;
Mustafa Khokha, Yale University;
Karen Liu, King's College London, UK;
Nanette Nascone-Yoder, North Carolina State University;
Ray Keller, University of Virginia;
Darcy Kelley, Columbia University;
Roberto Mayor, University College London, UK;
Andrea Wills, University of Washington;
Sarah Woolner, University of Manchester, UK;

For more information and application submission, please visit the website.
http://meetings.cshl.edu/courses.aspx?course=C-XENO&year=19


Thanks,
Lance Davidson, University of Pittsburgh
Chenbei Chang, University of Alabama


Course objectives:
Microinjection, microdissection, and grafts - Organizer, animal cap, neural crest and more. In situ hybridization, immunohistochemistry, CRISPR, and MO loss of function.
Germ cell transplants to raise CRISPR lines Homologous recombination in Xenopus with CRISPR. Live cell and embryo Imaging and fluorescence - confocal microscopy, image analysis and figure design. Genomic and proteomic approaches to development, regeneration, and disease models.