You can use this kit as a positive control e.g. when you start a WISH experiment anew. PFA-fixed embryos are provided in 100% ethanol. We estimate in situ hybridization using 10 fixed embryos as a single use; our kit provides sufficient amounts to repeat the procedure twice. You may download the protocol here.

  1. Japanese / English

The Web ordering system for this kit is being prepared. To obtain this kit, please ask the person below by e-mail.

The kit includes all the solutions described below.

  1. 1.PFA-fixed embryos (Stage 34-36), 10 embryos x 2

  2. 2.DIG-labeled antisense probe for detecting myod1 mRNA in hybridization buffer (1 µg/ml), 500 µl x 2

  3. 3.DIG-labeled sense probe for control in hybridization buffer (1 µg/ml), 500 µl x 2