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Living Resources

Requesting Strains, F1 Hybrids, Transgenics, Mutants

In this project, we provide the embryos, larvae, metamorphosed juveniles and adults of X. tropicalis as research resources. The living resources maintained at this resource center are roughly classified as follows. There is a list of living resources currently available at the bottom of the page. You can also select a derived lineage on the detail page.

  1. Inbred strains

  2. F1 hybrids between the inbred strains

  3. Transgenics

  4. Mutants

Advantages of hybrid frogs

We have maintained healthy inbred individuals after more than ten generations of sibling-matings. These resources are genetically and physiologically homogeneous and should be useful for genetics and genomics. However, hybrids of the different inbred strains are more robust and genetically homogeneous at colony level (= among individuals).

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  1. 09-01-2020

  2. Prices have been revised due to the increased costs associated with breeding and packaging. This revision includes the increase in the sales tax rate.

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Type: Wild type strain. Genetic modification: Unaltered.

This strain originated from a Nigerian strain maintained at the Grainger lab (University of Virginia, USA). Dr. Asashima (The University of Tokyo) obtained the sixth generation of the......(read more)

Reference: http://www.ncbi.nlm.nih.gov/pubmed/26222540/

https://xenopus.nbrp.jp/public/livingnogen.xhtml?line=0

Type: Mutant line. Genetic modification: Modified artificially.

Albino line with CRISPR/Cas9-targeted mutation in tyr gene.

Reference: http://www.ncbi.nlm.nih.gov/pubmed/27219625/

Type: Wild type strain. Genetic modification: Unaltered.

This strain was previously labelled as “Yasuda line” as Dr. K. Yasuda obtained X. tropicalis frogs from the Grainger lab in 1996 and propagated this colony for several generations. The......(read more)

Reference: http://www.ncbi.nlm.nih.gov/pubmed/26222540/

Type: Wild type strain. Genetic modification: Unaltered.

This strain was previously labelled as “Golden” and originally came from a stock maintained by Dr. E. Amaya at the University of California, Berkeley, and......(read more)

Reference: http://www.ncbi.nlm.nih.gov/pubmed/26222540/

https://xenopus.nbrp.jp/public/livingnogen.xhtml?line=5

Type: Wild type strain. Genetic modification: Unaltered.

This strain was taken from a wild population in Adipodoume, Ivory Coast, and has been shown to be diploid (Tymowska & Fishberg, 1973). Adult males and females were presented to Dr. K. Yoshizato (Institute for Amphibian Biology at that time) in......(read more)

Reference: http://www.ncbi.nlm.nih.gov/pubmed/26222540/

https://xenopus.nbrp.jp/public/livingnogen.xhtml?line=4

Type: Wild type hybrid. Genetic modification: Unaltered.

Hybrids of Nigerian A and Nigerian BH

Reference: http://www.ncbi.nlm.nih.gov/pubmed/26222540/

Type: Mutant line. Genetic modification: Modified artificially.

A model of human albinism, Hermansky-Pudlak Syndrome, with

CRISPR/Cas9-targeted mutation in hps6 gene. Transparent and useful for

live imaging analysis.


Please contact us for provision.


Reference: https://pubmed.ncbi.nlm.nih.gov/27595926/

Type: Transgenic line. Genetic modification: Modified artificially.

Pan-neuronal eGFP expression.

Reference: Sakagami, K., Igawa, T., Sakaguchi, Y. et al., in preparation.

https://xenopus.nbrp.jp/public/livingnogen.xhtml?line=14

Type: Mutant line. Genetic modification: Modified artificially.

Athymic line with TALEN-mediated targeted mutation in foxn1 gene. Absence of immunological rejection and useful for tissue transplantation experiments.

Reference: https://pubmed.ncbi.nlm.nih.gov/26847415/

List of living resources

Ordering systems were constructed by the department of NBRP Information.

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https://xenopus.nbrp.jp/public/livingnogen.xhtml?line=2
Order
https://xenopus.nbrp.jp/public/livingnogen.xhtml?line=1
https://xenopus.nbrp.jp/public/livingnogen.xhtml?line=12
https://xenopus.nbrp.jp/public/livingnogen.xhtml?line=10
https://xenopus.nbrp.jp/public/livingnogen.xhtml?line=13