1. Rearing Methods for Xenopus tropicalis/ Keiko Kashiwagi and Akihiko Kashiwagi (2017.3.6)

  2. Breeding methods run at the Amphibian Research Center are presented in the PDF document. It seems there are considerable differences in conditions such as water quality, temperature and day length, depending on the region. It is best to optimize your protocol to your conditions, referring to the methods described here rather than following strictly.

Dr. Keiko Kashiwagi giving a lecture on rearing methods in a technical training course

  1. Water Source of the Amphibian Research Center

  2. he main source of tap water at the Amphibian Research Center is the Ota River. Water from the Ota River is taken to the Senogawa Water Purification Plant, where it is purified and sent to Higashihiroshima (where the center is located). Hardness of the water in the clear reservoir of the plant is around 20 mg/mL, which is relatively low, even in Japan. Japanese tap waters are soft (≦ 120 mg/mL), even though. It is not known how suitable this hardness is for rearing X. tropicalis. The detailed water quality of the Senogawa plant can be seen on the Hiroshima Prefecture website (in Japanese). It can be accessed by entering “results of water quality tests” in Japanese in the search box of the top page.

  3. Rearing Q&A

  4. The two links below are linked to pages in Japanese. By clicking "into English by Google Translate" in the upper right of each page, you can read an English version produced by Google Translate.

  5. Why is the back of a tadpole bent? (in Japanese)

  6. Why are individuals much different in size after metamorphosis? (in Japanese)

  7. Please add your “how-to” on rearing X. tropicalis.

  8. Through this website, we want to publish useful “how-to” information from our users. You can contribute you “how-to” for rearing X. tropicalis by sending a message to the person in charge of the website (click “Contact Information” in the left side bar) or by posting to “BBS Everyone can Join”, in the Web Forum (click “Web Forum” in the left side bar).

Rearing -Equipment and Methods-

  1. You can do it at a cost of 100,000 yen!? How to make aquatic systems

  2. How to make aquatic systems for the rearing of X. tropicalis is presented here. They were devised and manufactured by Dr. Igawa, who is in charge of living resources, as a NBRP project. These systems are being implemented at NBRP sites. How to is originally described in Japanese. Although the three links below are linked to pages in Japanese, by clicking "Google Translation into English” at the upper right of each page, you can read an English version produced by Google Translate.

  3. When a study using these systems is published as a research paper, please acknowledge NBRP X. tropicalis and Dr. Igawa.

  1. Japanese / English

  1. Rearing